日時：　平成18年11月22日（水）　14:35〜



場所：　理学部Ｅ210



講師：　Miral Dizdaroglu博士

（National Institute of Standards and Technology, USA）



演題：　Oxidative damage to DNA: Mechanisms, measurement and disease



要旨：　Oxidative damage to DNA caused by oxygen-derived species produces numerous modifications in DNA of living organisms such as base and sugar lesions, 8,5'-cyclo-2'-deoxynucleosides, strand breaks and DNA-protein cross-links. We developed methodologies using gas chromatography (GC) or liquid chromatography (LC) coupled with mass spectrometry (MS) to simultaneously measure numerous products in cellular DNA with positive identification and accurate quantification. We studied oxidative DNA damage and its cellular repair in lung and breast cancers, xeroderma pigmentosum and Cockayne syndrome patients, in mice with knocked-out peroxiredoxin or neil1 genes, and in fish exposed to environmental pollutants. We showed the formation of DNA lesions after oxidative stress and lack of their cellular repair in cells from patients with lung cancer, breast cancer, xeroderma pigmentosum and Cockayne syndrome. We also showed the accumulation of DNA lesions in cellular DNA of mice due to gene knock-out and in DNA of fish exposed to environmental pollutants. The results of these studies will be presented and discussed in detail.

（　本セミナーは、英語で行われます。　）



≪本セミナーは４研究科共同セミナー認定科目です≫

連絡先：井出　博（理学研究科 数理分子生命理学専攻　内線：7457）