Biomaterials, tissue engineering, regenerative medicine, protein engineering, stem cell, cell-based microarray
We constructed antibody arrays for the quality control of mesenchymal stem cells. To ensure safe and efficient transplantation, the quality of graft cells must be controlled. We developed antibody arrays using a photo-assisted micro-patterning technique. The antibody arrays enable us to simply analyze the pattern of surface markers expressed in a cell population. The binding assay of test cells on the antibody array allows rapid characterization of cells more straightforward than conventional flow cytometry. We successfully applied this analytical technique to the characterization of mesenchymal stem cells. [ACS Appl. Mater. Interfaces, 2015, 7(30):16828−36].