Animal Reproduction


The Laboratory of Animal Reproduction conducts various studies on artificial manipulations of germ cells, and biochemical and molecular analyses of maturation, ovulation and fertilization of oocytes aiming at the improvement and preservation of livestock resources.

1) Artificial manipulation of germ cells (Maeda, T.)

2) Reproductive endocrinology (Shimada, M.)
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3) The development of reproductive technology to produce, select and utilize high-quality oocytes and embryos in mammal (Hoshino,Y.)


Sperm, Oocyte, Ovulation, Fertilization



Semen collection from Onagadori

Semen collection from Onagadori

Ovulating follicle

Ovulating follicle

Recent Publications

Eiman M-E. Aboagla, T. Maeda. (2011)Arbutin’s suppression of cryodamage in goat sperm and its mechanism of cryoprotection. Theriogenology, 76 (3): 538-546.

F. Kanyinji, T. Maeda. (2011)Individual plasma lipoprotein cholesterol levels in breeding Barred Plymouth Rock male chicken are influence by additional calcium in the diet. Journal of Animal and Veterinary Advances, 10 (11): 1394-1400.

F. Kanyinji, T. Maeda. (2010)Additional dietary calcium fed to Barred Plymouth Rock roosters reduces blood cholesterol, elevates seminal calcium, and enhances sperm motility, thermo-tolerance and cryosurvivability. Animal Reproduction Science, 120 (1): 158–165.

F. Kanyinji, T. Maeda, N. Araki. (2010)Effects of extra dietary calcium in form of fossil shells flour, or a mixture of fossil shells flour and fermented plant concentrate on growth performance, blood cholesterol levels, abdominal fat content and mechanical bone strength in broilers. Research Journal of Poultry Sciences, 3 (1): 11-18.

Kawashima, I., Liu, Z., Mullany, L.K., Mihara, T., Richards, J.S., Shimada, M. (2012) EGF-like factors induce expansion of the cumulus cell-oocyte complexes by activating calpain-mediated cell movement. Endocrinology 153: 3949-3959.

Noma, N., Kawashima, I., Fan, H.Y, Fujia, Y., Kawai, T., Tomoda, Y., Mihara, T., Richards, J.S., Shimada, M.(2011)LH induces Neuregulin 1 (NRG1) type III specific transcripts that interact with EGF-like factor pathways to control granulosa cell differentiation and oocyte maturation.Mol. Endocrinol. 25: 104-116.

Fujita, Y., Mihara, T., Okazaki, T., Shitanaka, M., Kushino, R., Ikeda, C., Negishi, H., Liu, Z., Richards, J.S., Shimada, M. (2011) Toll-like receptors (TLR) 2 and 4 on human sperm recognize bacterial endotoxins and mediate apoptosis. Hum. Reprod. 26: 2799-2806.

Fan, H.Y., Liu, Z., Shimada, M., Sterneck, E., Johnson, P.F., Hedrick, S.M., Richards, J.S. (2009)ERK1/2 in ovarian granulosa cells are critical for female fertility. Science, 324: 938-941.


Professor Maeda, T.
Professor Shimada, M.
Assistant Professor Hoshino, Y.



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