Ruthada CHANKLAN, Masaki MIZUNUMA, Ngampong KONGKATHIP, Komkrit HASITAPAN,Boonsong KONGKATHIP, and Tokichi MIYAKAWA
Identification of Saccharomyces cerevisiae Tub1 alpha-tubulin as a potential target for NKH-7, a cytotoxic 1-naphthol derivative compound.

Yoshifumi Kobayashi, Tomomi Inai, Masaki Mizunuma, Ichitaro Okada, Atsunori Shitamukai, Dai Hirata, and Tokichi Miyakawa
Identification of Tup1 and Cyc8 mutations defective in the responses to osmotic stress.

Ruthada Chanklan, Eiji Aihara, Saori Koga, Hidetoshi Takahashi, Masaki Mizunuma, and Tokichi Miyakawa
Inhibition of Ca2+-Signal-Dependent Growth Regulation by Radicicol in Budding Yeast.

S. Ohnuki, S. Nogami, H. Kanai, D. Hirata, Y. Nakatani, S. Morishita and Y. Ohya
Diversity of Ca2+-induced morphology revealed by morphological phenotyping of Ca2+-sensitive mutants of Saccharomyces cerevisiae.

K. Kume, T. Goshima, K. Miyahara, T. Toda and Dai Hirata
A method for Pmo25-associated kinase assay in fission yeast: The activity is dependent on two GC kinases Nak1 and Sid1.

T. Miyakawa and M. Mizunuma
Physiological roles of calcineurin in Saccharomyces cerevisiae with special emphasis on its roles in G2/M cell-cycle regulation.

G. Suzuki, H. Sawai, M. Ohtani, S. Nogami, F. Sano-Kumagai, A. Saka, M. Yukawa, T. L. Saito, J. Sese, D. Hirata, S. Morishita and Y. Ohya
Evaluation of image processing programs for accurate measurement of budding and fission yeast morphology.

K. Asakawa, K. Kume, M. Kanai, T. Goshima, K. Miyahara, S. Dhut, W. W. Tee, D. Hirata and T. Toda
The V260I mutation in fission yeast α-tubulin Atb2 affects microtubule dynamics and EB1-Mal3 localization and activates the Bub1 branch of the spindle checkpoint.

Hiroshi Yokoyama, Masaki Mizunuma, Michiyo Okamoto, Josuke Yamamoto, Dai Hirata, and Tokichi Miyakawa
Involvement of Yap1p downregulation in calcineurin-mediated G2 cell-cycle regulation in Saccharomyces cerevisiae.

Yoshifumi Kobayashi, Masaki Mizunuma, Hiroyuki Osada, and Tokichi Miyakawa
Identification of Saccharomyces cerevisiae ribosomal protein L3 as a target of curvularol, a G1-specific inhibitor of mammalian cells.

Farcasanu, I., Mizunuma, M., Nishiyama, F. & Miyakawa T.
Role of L-Histidine in Conferring Tolerance to Ni2⁺ in Saccharomyces cerevisiae Cells.

Mizunuma, M., Hirata, D. & Miyakawa, T.
Implication of Pkc1p protein kinase C in sustaining Cln2p level and polarized bud growth in response to calcium signaling in Saccharomyces cerevisiae.

Tutulan-Cunita, A. C., Ohnishi, T., Mizunuma, M., Hirata, D. & Miyakawa, T.
Involvement of Saccharomyces cerevisiae Pdr5p ATP-binding cassette transporter in calcium homeostasis.

Tutulan-Cunita, A. C., Mikoshi, M., Mizunuma, M., Hirata, D. & Miyakawa, T.
Mutational analysis of the yeast multidrug resistance ABC transporter Pdr5p with altered drug specificity.

K. Asakawa, M. Toya, M. Sato, M. Kanai, K. Kume, T. Goshima, M. A. Garcia, D. Hirata and T. Toda
Mal3, the fission yeast EB1 homolog, cooperates with Bub1 spindle checkpoint to prevent monopolar attachment.

M. Kanai, K. Kume, K. Miyahara, K. Sakai, K. Nakamura, K. Leonhard, D. J Wiley, F. Verde, T. Toda and D. Hirata
Fission yeast MO25 protein is localized at SPB and septum and is essential for cell morphogenesis.

Mizunuma, M., Miyamura, K., Hirata, D., Yokoyama, H. & Miyakawa, T.
Involvement of S-adenosylmethionine in G1 cell-cycle regulation in Saccharomyces cerevisiae.

Kubota, S., Takeo, I., Kume, K., Kanai, M., Shitamukai, A., Mizunuma, M., Miyakawa, T., Shimoi, H., Iefuji, H. & Hirata, D.
Effect of Ethanol on Cell Growth of Budding Yeast : Genes That Are Important for Cell Growth in the Presence of Ethanol.

A. Shitamukai, D. Hirata, Shinya Sonobe and T. Miyakawa
Evidence for antagonistic regulation of cell growth by the calcineurin and HOG pathways in Saccharomyces cerevisiae.

D. Hirata,N. Kishimoto,M. Suda,Y. Sogabe,S. Nakagawa,Y. Yoshida,K. Sakai,M. Mizunuma,T. Miyakawa,J. Ishiguro,T. Toda
Fission yeast Mor2/Cps12, a protein similar to Drosophila furry, is essential for cell morphogenesisi and its mutation induces Wee1-dependent G2 delay.

Y. Miyamoto,K. Machida,M. Mizunuma,Y. Emoto,N. Sato,K. Miyahara,D. Hirata,T. Usui,H. Takahashi,H. Osada,T. Miyakawa
Identification of Saccharomyces cerevisiae isoleucyl-tRNA synthetase as target of G1-specific inhibitor reveromycin A.

I. L. Ochoterena, D. Hirata, K. Kominami, J. Potashkin, F. Sahin, K. Wentz-Hunter, K. L. Gould, K. Sato, Y. Yoshida, L. Vardy and T. Toda
Conserved Wat1/Pop3 WD-repeat protein of fission yeast secures genome stability through microtubule integrity and may be involved in mRNA maturation.

O. Tanabe, D. Hirata, H. Usui, Y. Nishito, T. Miyakawa, K. Igarashi, and M. Takeda
Fission yeast homologues of the B’ subunit of protein phosphatase 2A: multiple roles in mitotic cell division and functional interaction with calcineurin.

Masaki, Mizunuma., Dai, Hirata., Rie, Miyaoka. & Tokichi, M.
GSK-3 kinase Mck1 and calcineurin coordinately mediate Hsl1 down regulation by Ca2+ in budding yeast.

M. Suda, S. Yamada, T. Toda, T. Miyakawa, and D. Hirata
Regulation of Wee1 kinase in response to protein synthesis inhibition.

Shitamukai, A., Mizunuma, M., Hirata, D., Takahashi, H. & Miyakawa, T.
A positive screening for drugs that specifically inhibit the Ca2+-signaling activity on the basis of the growth promoting effect on a yeast mutant with a peculiar phenotype.

I. C. Farcasanu, D. Hirata, E. Tsuchiya, K. Mizuta, and T. Miyakawa
Involvement of Thioredoxin Peroxidase Type II (Ahp1p) of Saccharomyces cerevisiae in Mn2+ homeostasis.

P. A. Radcliffe, D. Hirata, L. Yardy, and T. Toda
Functional dissection and hierarchy of tubulin-folding cofactor homologues in fission yeast.

S. Katayama, D. Hirata, M. Arellano, P. Perez, and T. Toda
Fission yeast α-glucan synthase Mok1 requires the actin cytoskeleton to localize the sites of growth and plays an essential role in cell morphogenesis downstream of protein kinase C function.

Suda, M., Fukui, M., Sogabe, Y., Sato, K., Morimatsu, A., Arai, R., Motegi, F., Miyakawa, T. Mabuchi, I. & Hirata, D.
Overproduction of elongation factor 1alpha, an essential translational component, causes aberrant cell morphology by affecting the control of growth polarity in fission yeast.

Cui, Z., Hirata, D. & Miyakawa, T.
Functional analysis of the promoter of the yeast SNQ2 gene encoding a multidrug resistance transporter that confers the resistance to 4-nitroquinoline N-oxide.

Mizunuma M., Hirata, D., Miyahara, K., Tsuchiya, E. & Miyakawa, T.
Role of calcineurin and Mpk1 in retulating the onset of mitosis in budding yeast.

P. A. Radcliffe, D. Hirata, D. Childs, L. Vardy, and T. Toda
Identification of novel temperature-sensitive lethal alleles in essential β-tubulin and nonessential α2-tubulin genes as fission yeast polarity mutants.

D. Hirata, H. Masuda, M. Eddison and T. Toda
Essential role of tubulin-folding cofactor D in microtubule assembly and its association with microtubules in fission yeast.

Hirata, D., Nakao, K., Fukui, M., Takenaka, H., Miyakawa, T. & Mabuchi, I.
Genes that cause aberrant cell morphology by overexpression in fission yeast : a role of a small GTP-binding protein Rho2 in cell morphogenesis.

Miyahara, K., Hirata, D. & Miyakawa, T.
Functional interaction of Isr1, a predicted protein kinase, with the Pkc1 pathway in Saccharomyces cerevisiae.

Cui, Z., Shiraki, T., Hirata, D. & Miyakawa, T.
Yeast gene YRR1, which is required for the resistance to 4-nitroquinoline N-oxide, mediates transcriptional activation of the multidrug resistance transporter gene SNQ2.

Farcasanu, I., Mizunuma, M., Hirata, D. & Miyakawa, T.
Involvement of histidine permease (Hip1p) in manganese transport in Saccharomyces cerevisiae.

Nakamura, T., Ohmoto, T., Hirata, D., Tsuchiya, E. and Miyakawa, T.
Yeast Crv4/Ttp1, a predicted type II membrane protein, is involved in an event important forgrowth, functionally overlapping with the event regulated by calcineurin-and Mpk1-mediated pathways.

Takeuchi, T., Miyahara, K., Hirata, D. and Miyakawa, T.
Mutational analysis of Yap1 protein, and AP-1-like transcriptional activator of Saccharomyces cerevisiae.

K. Miyahara, M. Mizunuma, D. Hirata and T. Miyakawa
The involvement of Saccharomyces cerevisiae multidrug resistance transporters encoded by PDR5 and SNQ2 genes in cation resistance.

Cui, Z., Hirata, D., Tsuchiya, E., Osada, H. and Miyakawa, T.
The multidrug resistance-related protein (MRP) sub-family (Yrs1/Yor1) of Saccharomyces cerevisiae is important for the tolerance to a broad range of organic anions.

Nakamura, T., Hirata, D., Tsuchiya E. and Miyakawa, T.
Genetic evidence for the functional redundancy of the calcineurin- and Mpk1-mediated pathways in the regulation of cellular events important for growth in Saccharomyces cerevisiae.

T. Toda, H. Niwa, T. Nemoto, S. Dhut, M. Eddison, T. Matsusaka, M. Yanagida and D. Hirata
The fission yeast sts5+ gene is required for maintenance of growth polarity and functionally interacts with protein kinase C and an osmosensing MAP-kinase pathway.

 K. Miyahara, D. Hirata and T. Miyakawa
yAP-1- and yAP-2-mediated, heat shock-induced transcriptional activation of the multidrug resistance ABC transporter genes in Saccharomyces cerevisiae.

Yaffe, M. P., Hirata, D., Verde, F., Eddison, M., Toda, T. and Nurse, P.
Microtubules mediate mitochondrial distribution in fission yeast.

D. Hirata, S. Harada, H. Namba and T. Miyakawa
Adaptation to high-salt stress in Saccharomyces cerevisiae is regulated by Ca2+/calmodulin-dependent phosphoprotein phosphatase (calcineurin) and cAMP-dependent protein kinase.

T. Matsusaka, D. Hirata, M. Yanagida and T. Toda
A novel protein kinase gene ssp1+ is required for alteration of growth polarity and actin localization in fission yeast.

T. Nakamura, T. Ohmoto, D. Hirata, E. Tsuchiya and T. Miyakawa
Cloning and characterization of the Saccharomyces cerevisiae gene SVS1 which encodes a serine- and threonine-rich protein required for vanadate resistance.

I. C. Farcasanu, D. Hirata, E. Tsuchiya, F. Nishiyama and T. Miyakawa
Protein phosphatase 2B of Saccharomyces cerevisiae is required for tolerance to manganese, in blocking the entry of ions into the cells.

K. Miyahara, D. Hirata and T. Miyakawa
Functional analysis of the promoter of the Saccharomyces cerevisiae multidrug resistant gene YDR1, which encodes a member of the ATP binding cassette (ABC) superfamily.

K. Hemmi, C. Julmanop, D. Hirata, E. Tsuchiya, J. Takemoto and T. Miyakawa
The physiological roles of membrane ergosterol as revealed by the phenotypes of syr1/erg3 null mutant of Saccharomyces cerevisiae.

D. Hirata, K. Yano and T. Miyakawa
Stress-induced transcriptional activation mediated by YAP1 and YAP2 genes that encode the Jun family of transcriptional activators in Saccharomyces cerevisiae.

D. Hirata, K. Yano, K. Miyahara and T. Miyakawa
Saccharomyces cerevisiae YDR1, which encodes a member of the ATP-binding cassette (ABC) superfamily, is required for multidrug resistance.

D. Mochizuki, K. Miyahara, D. Hirata, H. Matsuzaki, T. Hatano, S. Fukui, and T. Miyakawa
Overexpression and secretion of cellulolytic enzyme by δ-sequence-mediated multicopy integration of heterogous DNA sequences into the chromosomes of Saccharomyces cerevisiae.

T. Nakamura, Y. Liu, D. Hirata, H. Namba, S. Harada, T. Hirokawa and T. Miyakawa
Protein phosphatase 2B (calcineurin)-mediated, FK506-sensitive regulation of intracellular ions in yeast is an important determinant for adaptation to high salt stress conditions.   

H. Mukai, T. Kuno, H. Tanaka, D. Hirata, T. Miyakawa and C. Tanaka
Isolation and characterization of SSE1 and SSE2, new members of the yeast HSP70 multigene family.

D. Hirata, S. Aoki, K. Watanabe, M. Tsukioka and T. Suzuki
Stable overproduction of isoamyl alcohol by Saccharomyces cerevisiae with chromosome-integrated multicopy LEU4 genes.

D. Hirata, T, Miyakawa and I. Yamashita
Secretion of the glucoamylase-β-lactamase chimera protein by Saccharomyces cerevisiae.

D. Hirata, S. Aoki, K. Watanabe, M. Tsukioka and T. Suzuki
Breeding of a useful strain of sake yeast for ginjo-sake brewing.

D. Hirata and T. Hiroi
Genes that cause overproduction of isoamyl alcohol by increased gene-dosage effect in Saccharomyces cerevisiae.

D. Hirata, S. Fukui and I. Yamashita
Nucleotide sequence of the secretable protease gene PEP1 in the yeast Saccharomycopsis fibuligera.

I. Yamashita, D. Hirata, M. Machida and S. Fukui
Cloning and expression in Saccharomyces cerevisiae of the secretable acid protease gene from Saccharomycopsis fibuligera.