酵素化学

研究内容紹介

酵素の遺伝子発現や機能について主に研究している。特に、植物のビタミンCの機能と生合成・代謝、植物の細胞内輸送や有用な遺伝子組換え植物の作出に関して研究を行っている。

1)植物の細胞内輸送機構の解明と共に、植物を用いた有用物質の産生を行っている(藤川)。

2)環境ストレスに耐性をもつ植物の作出を目指し、植物の環境ストレスに対する応答や耐性機構について、分子レベルで解明する(末川)。

キーワード

遺伝子発現,遺伝子組換え植物,酵素,酸化ストレス,生合成機構,ビタミンC

 

 

スーパー植物の作出

スーパー植物の作出

遺伝子組換え植物

遺伝子組換え植物

最近の業績

Suekawa M, Fujikawa Y and Esaka M (2018) Two G-box–like elements essential to high gene expression of SlAKR4B in tomato leaves. Biosci Biotechnol Biochem. 82(3): 425-432

Suekawa M, Fujikawa Y, Inada S, Murano A and Esaka M (2016) Gene expression and promoter analysis of a novel tomato aldo-keto reductase in response to environmental stresses. J Plant Physiol. 200: 35-44

Fujikawa Y, Fujikawa R, Iijima N, Esaka M (2012) Characterization of secretory phospholipase A2 with phospholipase A1 activity in tobacco, Nicotiana tabacum (L.) Lipids, 47(3):303-312

Eltelib HA, Badejo AA, Fujikawa Y, Esaka M (2011) Gene expression of monodehydroascorbate reductase and dehydroascorbate reductase during fruit ripening and in response to environmental stresses in acerola (Malpighia glabra). J. Plant Physiol. 168(6):619-627

Badejo AA, Eltelib HA, Fukunaga K, Fujikawa Y, Esaka M. (2009) Increase in ascorbate content of transgenic tobacco plants overexpressing the acerola (Malpighia glabra) phosphomannomutase gene. Plant Cell Physiol. 50(2):423-428.

Tokunaga T, Miyata Y, Fujikawa Y, Esaka M. (2008) RNAi-mediated knockdown of XIP-type endoxylanase inhibitor gene, OsXIP, has no effect on grain development and germination in rice. Plant Cell Physiol. 49(7):1122-1127.

Oshima Y, Kamigaki A, Nakamori C, Mano S, Hayashi M, Nishimura M, Esaka M. (2008) Plant Catalase is Imported into Peroxisome by Pex5p but Distinct from Typical PTS1 Import. Plant Cell Physiol. 49(4):671-677.

Fujikawa Y, Kato N (2007) Split luciferase complementation assay to study protein-protein interactions in Arabidopsis protoplasts Plant J. 52(1):185-195.

Umemura Y., Ishiduka T., Yamamoto R., Esaka M. (2004) The Dof domain, a zinc finger DNA-binding domain conserved only in higher plants, truly functions as a Cys2/Cys2 Zn finger domain. Plant J., 37(5): 741-749.

Kamigaki A, Mano S, Terauchi K, Nishi Y, Tachibe-Kinoshita Y, Nito K, Kondo M, Hayashi M, Nishimura K, Esaka M (2003) Identification of peroxisoal targeting signal of pumpkin catalase and the binding analysis with PTS1 receptor. Plant J., 33 (1): 161-175.

スタッフ


up